Direct spectrophotometric assay of tryptophanase
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منابع مشابه
Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity
Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based on UV absorption is presented. The assay developed is based on the ligation of the α-hydroxy keton...
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The appropriate conditions for determining angiotensin-converting enzyme in human serum with use of 2-furanacryloyl-L-phenylalanylglycylglycine as substrate are described. The method, a modification of that reported by Holmquist et al. (Anal. Biochem. 95:540-548, 1979) for purified rabbit lung enzyme, is based on the blue shift of the absorption spectrum that occurs upon hydrolysis of the subst...
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The growing interest in measuring superoxide dismutase (EC 1.15.1.1) in many diseases calls for useful routine assays. For this purpose, the direct spectrophotometric method of Marklund (J Biol Chem 1976;251:7504-7) was improved to offer an alternative to the imprecise, indirect assays currently used. The decay of O2.- (from KO2) at pH 9.5 was monitored as the decrease in delta A (delta A = A25...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1976
ISSN: 0014-5793
DOI: 10.1016/0014-5793(76)80510-8